Competitive ELISA for Serodiagnosis of Bluetongue: A Refinement

Comparative Evaluation of c-ELISA and i-ELISA for Detection of Bluetongue Virus Antibodies in Sheep

Bluetongue (BT) is an infectious, noncontagious, arthropod-borne viral disease, principally of sheep but many domestic and wild animals are also affected by this disease (Akita et al., 1994). The severity of BT in sheep varies depending on the virus, the breed of sheep and environmental stress. Infection in cattle and goats is usually sub-clinical. Etiological agent of the disease belongs to th...

Validation of a Competitive ELISA for Detection of Babesia bigemina

Competitive Homogeneous Immunoassay for Rapid Serodiagnosis of Hantavirus Disease.

In this study, we describe a competitive homogeneous immunoassay that makes use of Förster resonance energy transfer (FRET) in rapid detection of pathogen-specific antibodies. The assay principle is based on competition between a monoclonal antibody (MAb) and serum antibodies to a given antigen. In the assay, named competitive FRET immunoassay (CFRET-IA), the FRET signal is induced if MAb carry...

Gluten fragment detection with a competitive ELISA.

The second generation of a competitive ELISA for prolamin quantification based on the R5 antibody was studied for method performance and suitability to detect partially hydrolyzed prolamins in food. To be able to convert signal intensities to gluten concentrations, as required by the Codex Alimentarius Standard, a new calibrator consisting of a peptic-tryptic digest of wheat, rye, and barley pr...

Serodiagnosis of bovine besnoitiosis by ELISA and immunofluorescence tests.

Sera from non-infected cattle and cattle infected with Anaplasma, Babesia, Theileria and Sarcocystis were tested for antibodies to Besnoitia in ELISA and immunofluorescence tests (IFT) with Besnoitia besnoiti of blue wildebeest origin as antigen. Only 2 out of 86 sera gave false positive reactions in ELISA and none in the IFT, indicating a high specificity for the tests. Three-hundred-and-three...